Contact
Positions
Professor, Assistant Dean for Professional Programs
- Organization:
- West Virginia University School of Medicine
- Department:
- Human Performance - Exercise Physiology
- Classification:
- Faculty
Education
- BS, Mammalian Physiology, University of California, San Diego, CA, 1991
- PhD, Nutritional Sciences, University of Wisconsin, Madison, WI, 2000
Publications
Publications (last 5 years)
2019
- Pinti, M.V., G.K. Fink, Q.A. Hathaway, A.J. Durr, A. Kunovac, and J.M. Hollander. Mitochondria dysfunction in type 2 diabetes mellitus: a systems approach. Am. J. Physiol. Endocrinol. Metab. 316:E268-E285, 2019.
- Hathaway, Q.A., A.J. Durr, D.L. Shepherd, M.V. Pinti, A.N. Brandebura, C.E. Nichols, A. Kunovac, W.T. Goldsmith, S.A. Friend, A.B. Abukabda, T.R. Nurkiewicz, and J.M. Hollander. miRNA-378a as a key regulator of cardiovascular health following engineered nanomaterial inhalation exposure. Nanotoxicology. 1:1-20, 2019.
- Hathaway, Q.A., S.M. Roth, M.V. Pinti, D.C. Sprando, A. Kunovac, A.J. Durr, C.C. Cook, G.K. Fink, T.B. Cheuvront, J.H. Grossman, G.A. Aljahli, A.D. Taylor, J.L. Allen and J.M. Hollander. Machine-learning to stratify diabetic patients using novel cardiac biomarkers and integrative genomics. Cardiovasc. Diabetol. 18:78, 2019.
- Kunovac, A., Q.A. Hathaway, M.V. Pinti, W.T. Goldsmith, A.J. Durr, G.K. Fink, T.R. Nurkiewicz, and J.M. Hollander. ROS promote epigenetic remodeling and cardiac dysfunction in offspring following maternal engineered nanomaterial (ENM) exposure. Part. Fibre. Toxicol. [in press].
- Geldenhuys, W.J., T. Long, P. Saralkar, T. Iwasaki, R. Nunez, R.R. Nair, M. Konkle, M. Menze, M.V. Pinti, J.M. Hollander, L. Hazelhurst, and A. Robart. Crystal structure of the mitochondrial protein mitoNEET bound to a benze-sulfonide ligand. Commun. Chem. [in press].
- Myers, M.J., D.L. Shepherd, A.J. Durr, D.S. Stanton, J.S. Mohamed, J.M. Hollander, and S.E. Always. The role of SIRT1 in skeletal muscle function and repair of older mice. J. Cachexia Sarcopenia Muscle. [in press].
2018
- Nichols, C.E., D.L. Shepherd, Q.A. Hathaway, A.J. Durr, D. Thapa, A. Abukabda, J. Yi, T.R. Nurkiewicz, and J.M. Hollander. Reactive oxygen species damage drives cardiac and mitochondrial dysfunction following acute nano-titanium dioxide inhalation exposure. Nanotoxicology. 12:32-48, 2018.
- Hathaway, Q.A., M.V. Pinti, A.J. Durr, S. Waris, D.L. Shepherd, and J.M. Hollander. Regulating microRNA expression: at the heart of diabetes mellitus and the mitochondrion. Amer. J. Physiol. Heart Circ. Physiol. 314:H293-H310, 2018.
- Stapleton, P.A., Q.A. Hathaway, C.E. Nichols, A.B. Abukabda, M.V. Pinti, D.L. Shepherd, C.R. McBride, J.M. Hollander and T.R. Nurkiewicz. Maternal engineered nanomaterial inhalation during gestation alters the fetal transcriptome. Part. Fibre Toxicol. 15:3-18, 2018.
- Chen, Q., M. Younus, J. Thompson, Y. Hu, J.M Hollander, and E.J. Lesnefsky. Intermediary metabolism and fatty acid oxidation: novel targets of electron transport chain driven injury during ischemia and reperfusion. Amer. J. Physiol. Heart Circ. Physiol. 314:H787-H795, 2018.
- Shepherd, D.L., Q.A. Hathaway, C.E. Nichols, A.J. Durr, M.V. Pinti, K.M. Hughes, A. Kunovac, S.M. Stine, and J.M. Hollander. Mitochondrial proteome disruption in the diabetic heart through targeted epigenetic regulation at the mitochondrial heat shock protein 70 (mtHsp70) nuclear locus. J. Mol. Cell. Cardiol. 119:104-115, 2018.
2017
- Pinti, M.V., Q.A. Hathaway, and J.M. Hollander. Role of microRNA in metabolic Shift during heart failure. Amer. J. Physiol. Heart Circ. Physiol. 312:H33-H45, 2017.
- Hathaway, Q.A., C.E. Nichols, D.M. Shepherd, P.A. Stapleton, S.L. McLaughlin, J.C. Stricker, M.V. Pinti, A.B. Abukabda, C.R. McBride, J. Yi, S.M. Stine, T.R. Nurkiewicz, and J.M. Hollander. Maternal engineered nanomaterial exposure disrupts progeny cardiac function and bioenergetics. Amer. J. Physiol. Heart Circ. Physiol. 312:H446-H458, 2017. [Featured as a podcast]
- Corbin, D.R., J.E. Rehg, D.L. Shepherd, Y.-M. Zhang, C.O. Rock, J.M. Hollander, S. Jackowski, and R. Leonardi. Excess coenzyme A reduces skeletal muscle performance and strength in mice overexpressing human PANK2. Mol. Genet. Metab. 120:350-362, 2017.
- Baradan, R., J.M. Hollander, and S. Das. Mitochondrial miRNAs in mitochondrial metabolism: just the tip of the iceberg. Can. J. Physiol. Pharmacol. 3:1-7, 2017.
- Shepherd, D.L., Q.A. Hathaway, M.V. Pinti, C.E. Nichols, A.J. Durr, S. Sreekumar, K.M. Hughes, S.M. Stine, I. Martinez, and J.M. Hollander. Exploring the mitochondrial microRNA pathway through Polynucleotide Phosphorylase (PNPase). J. Mol. Cell. Cardiol. 110:15-25, 2017.
2016
- Shepherd, D.L., C.E. Nichols, T.L. Croston, S.L. McLaughlin, A.B. Petrone, S.E. Lewis, D. Thapa, D.M. Long, G.M. Dick, and J.M. Hollander. Early cardiac dysfunction in the type 1 diabetic heart using speckle-tracking based strain imaging. J. Mol. Cell. Cardiol. 90:74-83, 2016.
2015
- Thapa, D., C.E. Nichols, S.E. Lewis, D.L. Shepherd, R. Jagannathan, T.L. Croston, K.J. Tveter, A.A. Holden, W.A. Baseler, and J.M. Hollander. Transgenic overexpression of mitofilin attenuates diabetes mellitus-associated cardiac and mitochondria dysfunction. J. Mol. Cell. Cardiol. 79:212-223, 2015.
- Stapleton, P.A., C.E. Nichols, J. Yi, C.R. McBride, V.C. Minarchick, D.L. Shepherd, J.M. Hollander, and T.R. Nurkiewicz. Microvascular and mitochondrial dysfunction in the female F1 generation after gestational TiO2 nanoparticle exposure. Nanotoxicology. 9:941-951, 2015.
- Jagannathan R., D. Thapa, C.E. Nichols, D.L. Shepherd, J.C. Stricker, T.L. Croston, W.A. Baseler, S.E. Lewis, I. Martinez I, and J.M. Hollander. Translational regulation of the mitochondrial genome following redistribution of mitochondrial microRNA (MitomiR) in the diabetic heart. Circ. Cardiovasc. Genet. 8:785-802, 2015.
- Nichols, C.E., D.L. Shepherd, T.L. Knuckles, D. Thapa, J.C. Stricker, P.A. Stapleton, V.C. Minarchick, A. Erdely, P.C. Zeidler-Erdely, S.E. Alway, T.R. Nurkiewicz, and J.M. Hollander. Cardiac and mitochondrial dysfunction following acute pulmonary exposure to mountaintop removal mining particulate matter. Amer. J. Physiol. Heart Circ. Physiol. 309:H2017-H2030, 2015.
- O’Connell, G.C., C.E Nichols, G. Guo, T.L. Croston, D. Thapa, J.M. Hollander, and E.E. Pistilli. IL-15Rα deficiency in skeletal muscle alters respiratory function and the proteome of mitochondrial subpopulations independent of changes to the mitochondrial genome. Mitochondrion. 25:87-97, 2015.
Additional Info
Research
To view Research Interests click HERE
About John Hollander
POSITIONS:
- 2012–present Vice Chair for Research
Division of Exercise Physiology
West Virginia University School of Medicine, Morgantown, WV - 2011–present Associate Professor (tenured)
West Virginia University School of Medicine, Morgantown, WV - 1/2009 – present Director of Graduate Studies, Division of Exercise Physiology
- 7/2005 - present Assistant Professor
West Virginia School of Medicine
Division of Exercise Physiology - 7/2004 - 7/2005 Assistant Project Scientist Step II
University of California, San Diego Department of Medicine
Supervisor – Wolfgang Dillmann, M.D. - 8/2000 - 7/2004 Postdoctoral Fellow
University of California, San Diego Department of Medicine
Supervisor – Wolfgang Dillmann, M.D.
PROFESSIONAL SOCIETIES:
- American Heart Association
- American College of Sports Medicine
- American College of Sports Medicine, Mid-Atlantic Chapter
- American Physiological Society
- International Society for Heart Research
- Society for Free Radical Biology and Medicine
- United Mitochondrial Disease Foundation
ACADEMIC AND PROFESSIONAL HONORS:
- 2010-present Named as Fellow of the American Heart Association, Council on BCVS
- 2009 West Virginia University Dean’s Award for Excellence in Research
- 3/1/05 - 6/30/05 Minority Investigator Research Supplement (MIRS)
NIH, National Heart, Lung, and Blood
“Cardiac Ischemia and Heat Shock Proteins” - 8/1/00 - 2/28/05 National Research Service Award Postdoctoral Traineeship
National Institute of Health - 7/1/98 - 6/30/99 American Heart Association Predoctoral Fellowship
American Heart Association
“Exercise and Antioxidant Enzyme Gene Regulation in Myocardium” - 9/1/98 - 11/30/98 Glenn Foundation/AFAR Scholarship
American Federation for Aging Research
“Aging and Antioxidant Enzyme Gene Regulation: Superoxide Dismutase Brought to Focus” - 9/1/98 - 5/31/99 Advanced Opportunity Fellowship
UW-Madison Graduate School - 1/1/97 - 5/31/98 Advanced Opportunity Fellowship
UW-Madison Graduate School - 1/1/94 - 12/31/96 National Research Service Award Predoctoral Traineeship
National Institute of Health - 9/1/93 - 1/1/94 Nutritional Sciences Fellowship
Interdepartmental Graduate Program Nutritional Science
Research Interests
Research Interests
The mitochondrion is an important organelle that is tasked with a multitude of functions requisite for cellular viability. As such, pathological influence on the mitochondrion remains at the forefront of research efforts focused on treating morbidity and mortality resulting from a multitude of disease states, including those influencing the cardiovascular system. Mechanistic evaluation of cardiovascular disease continues to suggest a central role for the mitochondrion, thus, understanding its contribution to the development and progression of cardiac pathologies is critical as we consider therapeutic options. The research in our laboratory focuses on understanding how different physiological and pathological states impact mitochondrial function, with the ultimate goal of developing therapeutic interventions designed to limit dysfunction to this critical organelle.
The Diabetic Heart
Diabetes mellitus is the world’s fastest growing disease with approximately 30 million people in the United States diagnosed and 84 million considered prediabetic (ADA). Cardiovascular complications including heart failure, remain the leading cause of mortality among diabetics. Our laboratory focuses on the development of treatments that can reduce bioenergetic deficits and contractile dysfunction associated with heart failure in diabetic patients through targeting interventions directed at the mitochondrion. Examination of mitochondria is complicated by the fact that two spatially-distinct mitochondrial subpopulations are present in the cardiac myocyte, interfibrillar mitochondria (IFM), which situate between the contractile proteins and subsarcolemmal mitochondria (SSM) that exist beneath the cell membrane (Figure A). These spatially-distinct mitochondrial subpopulations appear to be influenced differently during cardiovascular pathologies, suggesting that subcellular spatial location is essential for understanding disease progression and needs to be considered when developing therapeutic intervention. Our laboratory utilizes diseased human patient samples, cell culture systems, and unique genetically-modified mouse models to develop and test therapeutic approaches for treating the diabetic heart (Figure B). Cutting-edge genomic, (Figure C) and proteomic techniques are utilized to bioinformatically identify targets for therapeutic intervention. Emphasis is placed on understanding the role of non-coding RNAs (miRNA, lncRNA, YRNA) and epigenetic modifications driving gene regulation.
Environmental Stressors
Increases in cardiovascular events and death have been linked to the inhalation of environmental toxicants. Environmental stressors include particulate matter from unintended sources (ultrafine, PM2.5) and intended sources (engineered nanomaterial). Of particular concern are those that arise in the nanoscale range (Figure D) due to their increased surface area and physicochemical properties, which facilitate organ penetration. Despite their wide spread usage, it is unclear how particular matter exposure impacts human health from a consumer and occupational standpoint. Research from our laboratory indicates that acute and chronic exposure to engineered nanomaterials, negatively impacts cardiac contractile function and mitochondrial bioenergetics. Engineered nanomaterial exposure to the pregnant mother may interfere with fetal development (Figure E) and have acute and long-term cardiovascular consequences (Figure F) for the fetal pup that persist into adulthood. We are evaluating the impact of exposure to a number of nanomaterials (nano-TiO2, graphene, multi-walled carbon nanotubes).
In addition, particulate matter exposure generated by crustal material resulting from surface mining (mountaintop mining) (Figure G) which is prevalent in Appalachia, is associated with increased cardiovascular disease incidence. Our laboratory and others have reported extrapulmonary effects to cardiac and mitochondrial dysfunction following acute pulmonary exposure to particulate matter collected from sites proximal to active mountaintop mining operations in southern West Virginia (Figure H). Extrapulmonary effects of mountaintop mining particulate matter exposure is an active research priority for residents of Appalachia. Collaborations with the WVU School of Public Health and the local National Institute of Occupational Safety and Health (NiOSH) enable us to translate personal and occupational exposures of potentially toxic stressors into animal models, and determine the impact these particles may have on cardiac contraction and mitochondrial function.
Training Environment
The training environment in the Hollander Laboratory provides an opportunity for students and postdoctoral researchers to experience a broad array of techniques, skills and models. Numerous experimental models are utilized to explore biological questions and include cell culture systems, mammalian models of disease, human patient samples of clinical and disease relevance, and human induced pluripotent stem cells. Laboratory personnel participate in experiments that utilize molecular biology, biochemistry, physiology, and bioinformatic approaches. Cardiac contractile functional analyses are a particular focus in both in vivo (Video I) and in utero (Figure J) settings.
Laboratory staff participate in the generation of novel genetically-modified rodent models. The goal is to provide a training environment that facilitates a broadening of knowledge and skillset to increase competitiveness for future positions and employment. The cutting-edge techniques coupled with nature of the research topics provide students with a strong opportunity for independent research funding with institutions (National Institutes of Health) and private associations (American Heart Association) (Video K).
Current Laboratory
Previous Laboratory Pictures
Grants and Research
RESEARCH SUPPORT (ACTIVE)
-
NIH R01
National Heart, Lung and Blood Institute
“MicroRNA Regulation of the Mitochondrial Genome”
Role:PI
Dates:4/1/2017 – 3/31/20
Direct Costs:$899,945
The goal of these studies is to determine how mitochondrial microRNAs are regulated in diabetic human myocardium.
Whipkey Diabetes Mellitus Endowment
Community Foundation for the Ohio Valley
Whipkey Estate
“WVU HSC – Whipkey Trust Partnership”
Role: PI
Dates:7/1/2018 – 6/30/2019
Direct Costs:$118,000
This funds are an endowment that are used to support the mission of the WVU Mitochondria, Metabolism & Bioenergetics Working Group for which Dr. Hollander serves as the Director. This is a yearly endowment that is renewed annually.
WVU INBRE Next Generation Sequencing Grant
WVU INBRE IDEA
“Next Generation Sequencing of Human Endogenous Cardiac Stem Cells in the Human Diabetic Heart”
Role: PI
Dates:8/1/2018 – 7/31/2019
Direct Costs:$20,000
The goal of these studies is determine the mRNA and miRNA sequences of RISC bound mitochondrial genome encoded mRNAs in isolated mitochondria subpopulations using a HiSeq platform.
NIH R21
National Institute on Aging
“Activation of Mitochondrial Calpain Augments Cardiac Injury in Aged Hearts”
Role: Collaborating Investigator (PI: Q. Chen)
Dates:4/1/2017 – 3/31/2019
Direct Costs:$275,000
The goal of these studies is to understand the mechanisms driving calpains impact in the injured and aged heart with a focus on proteomic signature.
NIH R15
National Institute of Environmental Health and Safety
“PM2.5 from Fracking Operations Induces Microvascular and Mitochondrial Dysfunction”
Role: Collaborating Investigator (PI: T. Knuckles)
Dates:8/14/2017 – 8/13/2020
Direct Costs:$300,000
The goal of these studies is to determine the impact of ultrafine particulate matter arising from fracking operations on microvascular and mitochondrial functional endpoints.
NIH K99
National Institute of Environmental Health and Safety
“Mitochondrial Mechanisms, Microvascular Dysfunction and Gestational Nanotoxicology”
Role:Consultant (PI: P. Stapleton)
Dates:2/1/2015 – 1/31/2020
Direct Costs:$513,717
The goal of these studies is to determine how the mitochondrion in the vasculature is impacted by nanotoxicological insult during in utero exposure.
NIH R01
National Institute of Environmental Health and Safety
“Maternal Nanomaterial Exposures: Fetal Microvascular Endpoints and Programming”
Role: Collaborating Investigator (PI: T. Nurkiewicz)
Dates:8/28/2013 – 8/27/2022
Direct Costs:$1,250,000
The goal of these studies is to understand the impact of engineered nanomaterial exposures on microvascular function in progeny exposed in utero.
American Heart Association, Great Rivers Affiliate
American Heart Association
“Mitochondrial microRNA Import and Regulation”
Role: Sponsor (PI: Q. Hathaway)
Dates:8/1/2017 – 7/31/2019
Direct Costs:$53,688
The goal of these studies is determine the mechanisms regulating microRNA import into the mitochondrion and identify epigenetic mechanisms driving the process.
NIH R25
National Heart, Lung and Blood Institute
“The Summer Pharmacy Experiences in Academic Research (SPEAR) Program at Hampton University School of Pharmacy”
Role:Mentor
Dates:9/1/2016 – 8/31/2021
Direct Costs:$389,310
The overall goal of this training program is to foster desire in minority students to pursue a future in health-related research following graduation from pharmacy school.
RESEARCH SUPPORT (COMPLETED)
Whipkey Diabetes Mellitus Endowment
Community Foundation for the Ohio Valley
Whipkey Estate
“WVU HSC – Whipkey Trust Partnership”
Role: PI
Dates:3/1/2017 – 2/28/2018
Direct Costs:$110,000
This funds are an endowment that are used to support the mission of the WVU Mitochondria, Metabolism & Bioenergetics Working Group for which Dr. Hollander serves as the Director. This is a yearly endowment that is renewed annually.
NIH R56
National Heart, Lung and Blood Institute
“MicroRNA Regulation of the Mitochondrial Genome”
Role:PI
Dates:9/8/2015 – 8/31/18 (NCE)
Direct Costs:$274,662
The goal of these studies is to determine how mitochondrial microRNAs are regulated in diabetic human myocardium.
IGERT Training Fellowship
National Science Foundation
“Impact of Environmental Toxicity on Cardiac Function and Metabolism”
Role: Sponsor (PI: Q. Hathaway)
Dates:8/15/2015 – 7/31/2017
Direct Costs:$150,000
The goal of these studies is to determine the impact of environmental particulate exposure on cardiac function and metabolism while training the student in key research areas.
Clinical Pilot Funding Award
West Virginia Clinical and Translational Science Institute
“Therapeutic Manipulation of Mitochondrial Protein Import in the Type 2 Diabetic Patient Heart”
Role:PI
Dates: 7/1/2014 – 7/1/2016
Direct Costs:$75,000
The goal of these experiments is to test the potential therapeutic influence of manipulating the mitochondrial protein import system in type 2 diabetic patient tissue.
Research Funding and Development Grant
West Virginia University School of Medicine
“miRNA Regulation of the Mitochondrial Genome During Diabetes Mellitus”
Role:PI
Dates:4/1/2014 – 3/31/2015
Direct Costs:$25,000
The goal of these experiments is to determine the impact of diabetes mellitus on the regulation of the mitochondrial genome through miRNA interaction.
American Heart Association, Great Rivers Affiliate
American Heart Association
“Influence of Engineered Nanomaterial Inhalation on Cardiac Mitochondria”
Role: Sponsor (PI: C. Nichols)
Dates:7/1/2013 – 6/30/2015
Direct Costs:$52,000
The goal of these studies is determine the impact of nano-TiO2 exposure on cardiac mitochondria utilizing transgenic mouse models.
American Heart Association, Great Rivers Affiliate
American Heart Association
“Human and Mouse Type 2 Diabetes Mellitus and the Mitochondrial Proteome: The Beginning to Therapeutic Possibilities”
Role: Sponsor (PI: D. Shepherd)
Dates:7/1/2014 – 6/30/2015
Direct Costs:$26,000
The goal of these studies is determine the impact of type 2 diabetes mellitus on the mitochondrial proteome in mouse and patient models.
NIH DCC Pilot Award
National Institute of Diabetes, Digestive and Kidney Diseases
“MicroRNA Regulation in the Type 2 Diabetic Human Heart”
Role:PI
Dates:11/1-2013 – 10/31/2014
Direct Costs:$60,000
The goal of these studies is to determine the presence and contribution of mitochondrial microRNAs in human cardiac tissue from type 2 diabetic patients.
NIH R15
National Institutes on Aging
“VEGF and Skeletal Muscle Adaptation During Chronic Overload”
Role: Consultant (PI: K. Huey; Drake University)
Dates:8/8/2011 – 6/30/2014
Direct Costs:$300,000
The goal of these studies is discern the impact of aging on VEGF and heat shock protein 25 in skeletal muscle.
Graduate Training Grant (T32HL090610)
NIH, National Heart Lung and Blood Institute
“Research Training in Cardiovascular and Pulmonary Diseases”
Role: Sponsor Mentor (PI: S. Mustafa)
Dates: 8/1/2008 – 10/31/2014
The goal of this grant is to provide meritorious research and academic training for graduate students interested in pursuing research careers in the area of cardiovascular and pulmonary diseases.
SFRBM Research Mini-Fellowship
Society for Free Radical Biology and Medicine
“Genomic and Non-genomic Roles of STAT 3”
Role: Collaborating Investigator (PI: F. Zouein)
Dates:1/1/2013 – 12/31/2013
Direct Costs:$2,500
The goal of these studies is determine the genomic and non-genomic roles of STAT 3 and its regulation on reactive oxygen species.
WVU INBRE Next Generation Sequencing Grant
WVU INBRE IDEA
“Evaluation of Small RNAs in Mitochondrial Subpopulations”
Role: PI
Dates:10/3/2012 – 10/2/2013
Direct Costs:$10,000
The goal of these studies is determine the mRNA and miRNA sequences of RISC bound mitochondrial genome encoded mRNAs in isolated mitochondria subpopulations using a HiSeq platform.
WVU Genomics Next Generation Sequencing Grant
WVU Genomics Facility
“Evaluation of Small RNAs in Mitochondrial Subpopulations”
Role: PI
Dates:8/31/2012 – 10/31/2013
Direct Costs:$4,000
The goal of these studies is determine the mRNA and miRNA sequences of RISC bound mitochondrial genome encoded mRNAs in isolated mitochondria subpopulations using a MiSeq platform.
N30 Pharmaceuticals
“Evaluation of Nitrosoglutathione and its Metabolites in Lung Mitochondria”
Role:PI
Dates:11/1/2011 – 12/31/2013
Direct Costs:$6,000
The goal of these studies is determine the presence of nitrosoglutathione and its metabolites in isolated mitochondria.
NIH DP2
Director’s New Innovator Award, Type 1 Diabetes Pathfinder Award
National Institute of Diabetes, Digestive and Kidney Diseases
Mechanisms of Diabetic Cardiomyopathy: Mitochondria Subpopulations Brought to Focus
Role:PI
Dates:9/30/2008 – 8/31/2013
Direct Costs:$1,500,000
The goal of these studies is to design therapeutic interventions targeted to mitochondria that will aid in the treatment of type 1 diabetes mellitus.
NIH K99
National Institute on Aging
“Vascular Structure and Function with Aging, Viral Gene Therapy, and Exercise Training”
Role:Consultant (PI: B. Behnke)
Dates:9/1/2008 – 8/31/2013
Direct Costs:K-series Award
The goal of these studies is to test the broad hypothesis that aging induces microcirculatory dysfunction (both structural and functional) that impairs oxygen transport, and exercise training can attenuate this microcircular dysfunction.
IGERT Training Fellowship
National Science Foundation
“Acute Inhalation Exposure of Nano-Titanium Dioxide”
Role: Sponsor (PI: C. Nichols)
Dates:8/15/2012 – 8/14/2013
Direct Costs:$60,000
The goal of these studies is determine the impact of nano-titanium dioxide on mitochondrial disposition.
American Heart Association, Great Rivers Affiliate
“Examination of Protein Import in Mitochondria Influenced by Diabetes Mellitus in the Heart”
Role:Sponsor (PI: W. Baseler)
Dates:7/1/2010 – 6/30/2012
Direct Costs:$42,000
The goal of these studies is to determine the impact of type 1 diabetic insult on the mitochondrial import process in the heart.
American Heart Association Beginning Grant-In-Aid
American Heart Association, Great Rivers Affiliate
“Smoking and Thrombospondin-1 (TSP-1) Regulation of Skeletal Muscle Microvasculature”
Role:Collaborating Investigator (PI: I.M. Olfert)
Dates:7/1/2010 – 6/30/2012
Direct Costs:$121,000
The goal of these studies is to determine the impact of cigarette smoke TSP-1 on the skeletal muscle microvasculature density and function.
WVU Bridge Grant
WVU Research Corporation
“Diabetic Mitochondrial Dysfunction”
Role: PI
Dates:1/1/2012 – 12/31/2012
Direct Costs:$25,000
The goal of these studies is to determine the impact of type 1 diabetic insult on cardiac mitochondrial protein import machinery and generate preliminary data for an NIH R01 resubmission.
American Heart Association Beginning Grant-In-Aid
American Heart Association, Great Rivers Affiliate
“Cardiac-Specific Inhibition of Myostatin in Hypertrophy of the Adult and Aging Heart”
Role:Collaborating Investigator (PI: M. Morissette)
Dates:7/1/2009 – 6/30/2011
Direct Costs:$121,000
The goal of these studies is to determine the role of myostatin inhibition in the aged and adult heart.
NIH DP2
Director’s New Innovator Award, Type 1 Diabetes Pathfinder Award (Administrative Supplement)
National Institute of Diabetes, Digestive and Kidney Diseases
Mechanisms of Diabetic Cardiomyopathy: Mitochondria Subpopulations Brought to Focus
Role:PI
Dates:1/1/2010 – 9/30/2010
Direct Costs:$85,000
The goal of these studies is to design therapeutic interventions targeted to mitochondria that will aid in the treatment of type 1 diabetes mellitus. The goal of the supplement is to provide equipment for infrastructure.
NIH DP2
Director’s New Innovator Award, Type 1 Diabetes Pathfinder Award (Travel Supplement)
NIH, National Institute of Diabetes, Digestive and Kidney Diseases
Mechanisms of Diabetic Cardiomyopathy: Mitochondria Subpopulations Brought to Focus
Role:PI
Dates:1/1/2010 – 9/30/2010
Direct Costs:$1,500
The goal of these studies is to design therapeutic interventions targeted to mitochondria that will aid in the treatment of type 1 diabetes mellitus. The goal of the supplement is to provide for travel to the NIDDK New Principal Investigators Workshop.
NIH P30
National Cancer Institute
“Shared Instrumentation: In Vivo Ultrasound”
Role:Project Leader (PI: K. Martin)
Dates:1/1/2010 – 9/30/2010
Direct Costs:$500,000
The goal of this grant is to obtain a Vevo 2100 ultrasound machine. The goal of Dr. Hollander’s project is to utilize the piece of equipment for cardiac contractile analyses in diabetic mice as well as genetically-altered diabetic mice.
American Heart Association Grant-In-Aid
American Heart Association, Great Rivers Affiliate
“Mitochondrial Subpopulation Response to Ischemia/Reperfusion Injury: Influence of mPHGPX Overexpression”
Role:PI
Dates:7/1/2008 – 6/30/2010
Direct Costs:$121,000
The goal of these studies is to determine the differential response of mitochondria populations to ischemia/reperfusion injury, and determine the efficacy of mPHGPx overexpression.
American Heart Association Predoctoral Fellowship
American Heart Association, Great Rivers Affiliate
“Influence of a Diabetic Phenotype on Specific Mitochondrial Subpopulations in the Heart”
Role:Sponsor (PI: E. Dabkowski)
Dates:7/1/2008 – 6/30/2010
Direct Costs:$42,000
The goal of these studies is to determine the differential response of mitochondria populations to diabetic insult, and determine the efficacy of mPHGPx overexpression.
Research Funding and Development Grant
West Virginia University School of Medicine
“Cardiac-Specific Inhibition of Myostatin in Hypertrophy of the Adult and Aging Heart”
Role:Collaborating Investigator (PI: M. Morissette)
Dates:7/1/2009 – 6/30/2010
Direct Costs:$25,000
The goal of these experiments is to determine the impact on cardiac function of heat shock protein 25 removal in various cell types, using a novel conditional knockout animal generated by the PI.
American Heart Association Beginning Grant-In-Aid
American Heart Association
“Myocardial Protection from I/R Injury by mPHGPx Overexpression”
Role: PI
Dates:7/1/2006 – 6/30/2008
Direct Costs:$121,000
The goal of these experiments is to determine the impact adenoviral transgene delivery of mPHGPx overexpression on cardiac function during ischemia/reperfusion insult.
Amyotrophic Lateral Sclerosis Association Research Grant
ALS Society
“Mutant Superoxide Dismutase-Induced Mitochondrial Defects”
Role:Collaborating Investigator (PI: M. Gunther)
Dates:2/1/2007 – 1/31/2008
Direct Costs:$40,000
The goal of these experiments is to determine the impact of SOD2 on mitochondrial defects associated with ALS. My role as Collaborating Investigator is to generate adenoviral particles expressing SOD2 and specific mutant SOD2 proteins that are targeted to the mitochondrion to gain insight into their effect on disease progression.
Research Funding and Development Grant
West Virginia University School of Medicine
“Cell-Type Specific Removal of Hsp25 in the Diabetic Heart”
Role:PI
Dates:1/1/2007 – 12/31/2007
Direct Costs:$25,000
The goal of these experiments is to determine the impact on cardiac function of heat shock protein 25 removal in various cell types, using a novel conditional knockout animal generated by the PI.
Research Funding and Development Grant
West Virginia University School of Medicine
“Mitochondrial Protection from I/R Injury by mPHGPx Overexpression”
Role:PI
Dates:1/1/2006 – 12/31/2006
Direct Cost:$25,000
The goal of these experiments is to determine the impact adenoviral transgene delivery of mPHGPx overexpression on cardiac function during ischemia/reperfusion insult.
Minority Investigator Research Supplement (MIRS)
NIH, National Heart, Lung, and Blood
“Cardiac Ischemia and Heat Shock Proteins”
Role: PI
Dates:1/1/2005 – 7/1/2005
Direct Costs:$110,000
The goal of these experiments is to determine the combinatorial effect of adenoviral overexpression of multiple heat shock protein constructs, on protection from ischemia/reperfusion insult.
Patient Care Information
Special Training
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Fellowship, Medicine
University of California, San Diego, CA, 2005