Kathy Brundage, PhD

Director, WVU Flow Cytometry & Single Cell Core Facility
Research Assistant Professor

Organization:

West Virginia University School of Medicine

Department:

Microbiology, Immunology & Cell Biology

Classification:

Faculty

Member

Organization:

West Virginia University WVU Cancer Institute

Department:

WVU Cancer Institute Research Programs

Classification:

Staff

• BS, Quinnipiac University, 1985
• PhD, University of Pennsylvania, 1994

Selected References

Klinke DJ, Brundage KM. Scalable analysis of flow cytometry data using R/Bioconductor. Cytometry A. 2009;75(8):699-706. PMCID: PM2941976

Hanson ML, Brundage KM, Schafer R, Tou JC, Barnett JB. Prenatal cadmium exposure dysregulates sonic hedgehog and Wnt/beta-catenin signaling in the thymus resulting in altered thymocyte development. Toxicol.Appl.Pharmacol. 2010;242(2):136-45. PMCID: PMC2789900.

Hanson ML, Peer CJ, Brundage R, Callery PS, Brundage K, Schafer R, Eremin S, Barnett JB. Subcellular localization of the amide class herbicide 3,4-dichloropropionanilide (DCPA) in T cells and hepatocytes. J. Toxicol. Environ. Health A 2010: 73(1):1-4. PMCID: PMC2789900

Hanson ML, Brundage KM, Schafer R, Tou JC, Barnett JB. Prenatal cadmium exposure dysregulates sonic hedgehog and Wnt/beta-catenin signaling in the thymus resulting in altered thymocyte development. Toxicol.Appl.Pharmacol. 2010;242(2):136-45. PMCID: PMC2789900.

Zellner LC, Brundage KM, Hunter DD, Dey RD. Early Postnatal Ozone Exposure Alters Rat Nodose and Jugular Sensory Neuron Development. Toxicol.Environ.Chem. 2011;93(10):2055-71. PMCID: PMC3226817.

Hamadani M, Gibson LF, Remick SC, Wen S, Petros W, Tse W, Brundage KM, Vos JA, Cumpston A, Bunner P, Craig MD. Sibling donor and recipient immune modulation with atorvastatin for the prophylaxis of acute graft-versus-host disease. J Clin Oncol. 2013 Dec 10;31(35):4416-23.

Hanson ML, Holásková I, Elliott M, Brundage KM, Schafer R, Barnett JB. Prenatal cadmium exposure alters postnatal immune cell development and function. Toxicol Appl Pharmacol. 2012 Jun 1;261(2):196-203. 

View more at PubMed

In my current position as technical director of the WVU Flow Cytometry Core Facility, I am responsible for the day to day operations of the facility including the maintenance of the instruments, billing for use of the facility, running samples, analyzing data, training users to run their own samples and analyze their data. In addition, I provide consultation on experimental design and reagent purchases for those who wish to use flow cytometry in their research.

In my capacity as technical director of the WVU Flow Cytometry Core Facility I work with many laboratories across the WVU campus. Recently, there were three research projects that I provided significant input resulting in authorship on two papers and an abstract. Working with Leor Zellner (graduate student) in Dr. Richard Dey’s lab, we developed an assay in which she could identify and count the neurons that innervate the lungs. This work was published in the Zellner et al paper listed below. I also assisted Dr. John Barnett’s laboratory, in particular Miranda Hanson (graduate student) and Ida Holaskova (post-doctoral fellow), in developing a panel of antibodies to phenotype the different cell populations in the thymus and spleen and for isolating these cells via flow cytometry sorting. This work was published in the Hanson et al papers listed below. Finally, I have worked closely with Dr. Adam Halasz to generate the data for his mathematical model of VEGF binding to its receptor. This work has resulted in an abstract which was presented at a scientific meeting.

In addition to my work at WVU, I have also worked with individuals at other institutions as a member of the Antibody Technology Research Group (ARG) of ABRF. The purpose of this group is to collect and share knowledge about generating, producing, purifying, fragmenting and conjugating antibodies with the larger scientific community. In addition, this group is responsible for advancing and evaluating the technologies used within the antibody field (i.e. rabbit monoclonal antibodies, phage display technology). Currently, I am working with other members of ARG to generate an updated antibody manual that will assist members of the research community in the generation, screening and choosing monoclonal and polyclonal antibodies for their research.

Curriculum Vitae

Education

1985
BS in Medical Technology from Quinnipiac University in Hamden, CT

1994
PhD in Immunology from University of Pennsylvania in Philadelphia, PA

1994-1999
Post-doctoral Fellow at Thomas Jefferson Medical College in Philadelphia, PA

1999-2001
Post-doctoral Fellow at West Virginia University in Morgantown, WV

Grant Support

P20 RR032138 CoBRE for Signal Transduction and Cancer Phase III
Funding period: 07/15/11-07/14/16
Role: Co-I Flow Cytometry Core Technical Director
The goals of this project are to nurture collaborative interactions, support core facilities, and mentor junior scientists involved in studies of signal transduction in cancer.

Presentations

May 2010. WVU flow cytometry core facility. Obsorne Research Group of MBRCC. Morgantown, WV

August, 2011. WVU flow cytometry core facility. 2nd Annual Alexander B. Obsorne Hematopoietic Malignancy and Transplantation Program Retreat. Morgantown, WV

Current Appointments

7/2007 – present
Technical Director - WVU Flow Cytometry Facility

2/2001 – present
Research Assistant Professor - Dept. Micro/Immuno/Cell Bio

1/2010 – present
Associate Member - MBRCC

Non-Programmatically Aligned Cancer Center Member