Andrey A. Bobko, BS, MSc, PhD

Research Assistant Professor

Organization:

West Virginia University School of Medicine

Department:

Biochemistry

Classification:

Faculty

Member

Organization:

West Virginia University WVU Cancer Institute

Department:

WVU Cancer Institute Research Programs

Classification:

Faculty

Most Recent:

A combined positron emission tomography (PET)- electron paramagnetic resonance imaging (EPRI) system: initial evaluation of a prototype scanner.
Tseytlin M, Stolin AV, Guggilapu P, Bobko AA, Khramtsov VV, Tseytlin O, Raylman RR.
Phys Med Biol. 2018; 63(10): 105010.
PMC5979266

Triarylmethyl-based biradical as a superoxide probe.
Poncelet M, Driesschaert B, Bobko AA, Khramtsov VV.
Free Radic Res. 2018; 52(3): 373-379.
PMC5835163

LPA signaling is regulated through the primary cilium: a novel target in glioblastoma.
Loskutov YV, Griffin CL, Marinak KM, Bobko AA, Margaryan NV, Geldenhuys WJ, Sarkaria JN, Pugacheva EN.
Oncogene. 2018; 37(11): 1457-1471.
PMC5854509

Oxygen-induced leakage of spin polarization in Overhauser-enhanced magnetic resonance imaging: application for oximetry in tumors.
Gorodetskii AA, Eubank TD, Driesschaert B, Poncelet M, Ellis EJ, Khramtsov VV, Bobko AA.
J Magn Reson. 2018; 297: 42-50.
PMC6289650

In Vivo EPR assessment of pH, pO2, redox status, and concentrations of phosphate and glutathione in the tumor microenvironment.
Bobko AA, Eubank TD, Driesschaert B, Khramtsov VV.
J Vis Exp. 2018; (133).
PMC5905398

Exchange phenomena in the electron paramagnetic resonance spectra of the nitroxyl and trityl radicals: multifunctional spectroscopy and imaging of local chemical microenvironment.
Khramtsov VV, Bobko AA, Tseytlin MP, Driesschaert B.
Anal Chem. 2017; 89(9): 4758-4771.
PMCID5513151

Nitro-triarylmethyl radical as dual oxygen and superoxide probe.
Driesschaert B, Bobko AA, Khramtsov VV, Zweier JL.
Cell Biochem Biophys. 2017; 75(2): 241-246.
PMC5116421

Concurrent longitudinal EPR monitoring of tissue oxygenation, acidosis, and reducing capacity in mouse xenograft tumor models.
Bobko AA, Evans J, Denko NC, Khramtsov VV.
Cell Biochem Biophys. 2017; 75(2): 247-253.
PMC5116002

Interstitial inorganic phosphate as a tumor microenvironment marker for tumor progression.
Bobko AA, Eubank TD, Driesschaert B, Dhimitruka I, Evans J, Mohammad R, Tchekneva EE, Dikov MM, Khramtsov VV.
Sci Rep. 2017; 7: 41233.
PMC5259743

Poly-arginine conjugated triarylmethyl radical as intracellular spin label.
Driesschaert B, Bobko AA, Eubank TD, Samouilov A, Khramtsov VV, Zweier JL.
Bioorg Med Chem Lett. 2016; 26(7): 1742-1744.
PMC4807691

Redox properties of the nitronyl nitroxide antioxidants studied via their reactions with nitroxyl and ferrocyanide.
Bobko AA, Khramtsov VV.
Free Radic Res. 2015; 49(8): 919-926.
PMC4718613
 

Biomedicine

Primary Project

The importance of the tumor microenvironment (TME) in tumor progression, invasion, and therapy is widely recognized. Intermittent and hypoxic oxygenation (pO₂) and extracellular acidosis (pH_e) of tumor tissues are among the most established hallmarks in solid TME, while extracellular inorganic phosphate (Pi) has been recently identified as a new signaling molecule of importance in tumorigenesis (marker of metastatic potential).

The α_vβ₃ and α_vβ₅ integrins are usually expressed at low levels in most adult epithelia but can be highly upregulated in some tumors. These integrins are involved in angiogenesis and metastasis of solid tumors, and their inhibition resulted in significant reduction of functional vessel density, retardation of tumor growth, and metastasis in vivo.

In this project we propose to develop theranostic multifunctional paramagnetic trityl probes for in vivo monitoring of basic physiological parameters (pO₂, pH, Pi) using electron paramagnetic resonance-based techniques and patient-derived tumor xenograft (PDX) models.

Proposed trityl probes are designed to have minimal toxicity, a wide range of pharmacokinetic time, and antiangiogenic therapeutic effects due to high affinity of the probes to the α_vβ₃ integrins in tumor tissue.

The best probe formulations are used to construct a real-time TME profile during tumor development and antiangiogenic treatment using theranostic

We hypothesize that the TME signature can predict levels of success for α_vβ integrins based on antiangiogenic therapy. Successful completion of this proposed project will result in development of new theranostic paramagnetic multifunctional probes which can be easily modified with appropriate targeting motif to study drug therapeutic effects.